客户科研成果列表


基因合成 | 基因克隆 | sgRNA文库 | 密码子优化| 引物合成| 探针合成| 测序| 载体构建| DNA存储| RNA干扰

基因合成

  1. Jinghua W., et al. CAR-T cells targeting CLL-1 as an approach to treat acute myeloid leukemia. Journal of Hematology & Oncology, 2018. 11:7. Doi: 10.1186/s13045-017-0553-5.
  2. Gong S., Wu C. Generation of Fabs-in-Tandem Immunoglobulin Molecules for Dual-Specific Targeting. Methods, Aug 3, 2018. pii: S1046-2023(18)30105-1. doi: 10.1016/j.ymeth.2018.07.014.
  3. Xiu, H., et al., microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147. Journal of Cancer. 2018; 9(15):2603-2611. doi:10.7150/jca.24918.
  4. Yang, X., et al., Expression of phosphotransacetylase in Rhodosporidium toruloides leading to improved cell growth and lipid production. Royal Society of Chemistry, 10th July 2018. 8:24673-24678. doi: 10.1039/C8RA03028F.
  5. Shi, J., et al., A novel QconCAT-based proteomics method for determining allele-specific protein expression (ASPE): a new approach to identify cis-acting genetic variants. BioRxiv, 2018. doi: 10.1101/264614.
  6. Su, J., et al., Galectin-10: a new structural type of prototype galectin dimer and effects on saccharide ligand binding. Glycobiology, 2018. 28(3): p. 159-168. doi:0.1093/glycob/cwx107
  7. Wang, X., et al., Visualizing soluble protein mutants by using monomeric red fluorescent protein as a reporter for directed evolution. Applied Biochemistry and Biotechnology, 2018. 185(1): p.81-90. doi: 10.1007/ s12010-017-2640-z.
  8. Lu, H., et al., Identification of a L-Lactate dehydrogenase with 3, 4-dihydroxyphenylpyruvic reduction activity for L-Danshensu production. Process Biochemistry, 2018. doi: 10.1016/j.procbio.2018.06.011
  9. Jin, W., et al., Increased soluble heterologous expression of a rat brain 3-O-sulfotransferase 1 – A key enzyme for heparin biosynthesis. Protein Expression and Purification, 2018, Vol.151, P.23-29. doi: 10.1016/j.pep.2018.06.007
  10. Li J, Zhang Y, Zhang Y, et al. GSNOR modulates hyperhomocysteinemia-induced T cell activation and atherosclerosis by switching Akt S-nitrosylation to phosphorylation[J]. Redox biology, 2018. doi.org/10.1016/j.redox.2018.04.021
  11. Cheng Y, Chen G, Wang L, et al. Triptolide-induced mitochondrial damage dysregulates fatty acid metabolism in mouse sertoli cell s[J]. Toxicology letters, 2018, 292: 136-150. doi: 10.1016/j.toxlet.2018.04.035.
  12. Pan Y, Sun H, Hu X, et al. The inhibitory role of miR 485 5p in colorectal cancer proliferation and invasion via targeting of CD147 [J]. Oncology reports, 2018, 39(5): 2201-2208. doi: 10.3892/or.2018.6309.
  13. Zhang W, He Z, Yi L, et al. A dual-functional microfluidic chip for on-line detection of interleukin-8 based on rolling circle amplification[J]. Biosensors and Bioelectronics, 2018, 102: 652-660. doi: 10.1016/j.bios.2017.12.017.
  14. Chang Z, Meng F, Zhang Z, et al. MicroRNA‐193b‐3p regulates matrix metalloproteinase 19 expression in interleukin‐1β‐induced human chondrocytes[J]. Journal of cellular biochemistry, 2018, 119(6): 4775-4782. doi: 10.1002/jcb.26669.
  15. He, P., et al., Development and application of a duplex real-time RT-PCR assay for simultaneous detection of coxsackievirus A6 and coxsackievirus A10. Annals of Clinical and Laboratory Science, 2017. 47(2): p. 178-183. PMID: 28442520
  16. Wang, Li., et al., Increased processivity, misincorporation, and nucleotide incorporation efficiency in Sulfolobus solfataricus Dpo4 thumb domain mutants. Applied and Environmental Microbiology, 2017. 83(18): p. e01013-17. doi: 10.1128/AEM.01013-17.
  17. Wang, X., et al., Engineering Escherichia coli nicotinic acid mononucleotide adenylyltransferase for fully active amidated NAD biosynthesis. Applied and Environmental Microbiology, 2017. 83(13): p. e00692-17. doi: 10.1128/AEM.00692-17.
  18. Xie, Z., et al., Design and chemical synthesis of eukaryotic chromosomes. Chemical Society Reviews, 2017. 46(23): p. 7191-7207. doi:10.1039/ c7cs00208ds
  19. Dai, S., et al., HNRNPA2B1 regulates the epithelial-mesenchymal transition in pancreatic cancer cells through the ERK/snail signalling pathway. Cancer Cell Int, 2017. 17: p. 12. doi: 10.1186/s12935-016-0368-4.
  20. Jin, L., et al., Icaritin induces mitochondrial apoptosis by up-regulating miR-124 in human oral squamous cell carcinoma cells. Biomed Pharmacother, 2017. 85: p. 287-295. doi: 10.1016/j.biopha.2016.11.023.
  21. Li, N., et al., A DNA-directed covalent conjugation fluorescence probe for in vitro detection of functional matrix metalloproteinases. Analyst, 2017. doi: 10.1039/C6AN02339H
  22. Liu J, Chen W, Zhang H, et al. miR 214 targets the PTEN mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer[J]. Oncology letters, 2017, 14(5): 5711-5718. doi: 10.3892/ol.2017.6953
  23. Duan L, Ding W, Liu X, et al. Biosynthesis and engineering of kaempferol in Saccharomyces cerevisiae[J]. Microbial cell factories, 2017, 16(1): 165. doi: 10.1186/s12934-017-0774-x
  24. Zhao Z, Xue J, Song Y, et al. Downregulated long non coding RNA TCONS_00068220 upregulates apoptosis in gastric cancer cells[J]. Oncology letters, 2017, 14(5): 6143-6150. doi: 10.3892/ol.2017.6977
  25. Si, X., et al., Upregulation of miR-99a is associated with poor prognosis of acute myeloid leukemia and promotes myeloid leukemia cell expansion. Oncotarget, 2016. 7(47): p. 78095-78109. doi: 10.18632/ oncotarget.12947.
  26. Na, R., et al., Indentification of vincamine indole alkaloids producing endophytic fungi isolated from Nerium indicum, Apocynaceae. Microbiol Res, 2016. 192: p. 114-21. doi: 10.1016/j.micres.2016.06.008.
  27. Pan, X., et al., Physcion induces apoptosis in hepatocellular carcinoma by modulating miR-370. Am J Cancer Res, 2016. 6(12): p. 2919-2931. PMCID: PMC5199765
  28. Li, Z., et al., NS5ATP6 modulates intracellular triglyceride content through FGF21 and independently of SIRT1 and SREBP1. Biochem Biophys Res Commun, 2016. 475(1): p. 133-9. doi: 10.1016/j.bbrc.2016.05.056.
  29. Yang, H., et al., Optimized expression of prolyl aminopeptidase in Pichia pastoris and its characteristics after glycosylation. World J Microbiol Biotechnol, 2016. 32(11): p. 176. doi: 10.1007/s11274-016-2135-z.
  30. Tang, Y., et al., Radiation-induced miR-208a increases the proliferation and radioresistance by targeting p21 in human lung cancer cells. J Exp Clin Cancer Res, 2016. 35: p. 7.doi: 10.1186/s13046-016-0285-3
  31. Zhi N, Wan Z, Liu X, et al. Codon optimization of human parvovirus B19 capsid genes greatly increases their expression in nonpermissive cells[J]. Journal of virology, 2010, 84(24): 13059-13062.doi: 10.1128/JVI.00912-10

基因克隆

  1. Hu, J., et al., Clone and functional analysis of Seryl-tRNA synthetase and Tyrosyl-tRNA synthetase from silkworm, Bombyx mori. Sci Rep, 2017. 7: p. 41563. doi: 10.1038/srep41563.
  2. Yan, H., et al., CRIF1 enhances p53 activity via the chromatin remodeler SNF5 in the HCT116 colon cancer cell lines. BBA – Gene Regulatory Mechanisms.doi: 10.1016/j.bbagrm

sgRNA文库

  1. Lu, Y., et al., Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System. Mol Plant, 2017.doi: 10.1016/j.molp.2017.06.007.

密码子优化

  1. Liu, B., et al., Codon optimization significantly enhanced the expression of human 37-kDa iLRP in Escherichia coli. Biotech, 2018. 8(4): p. 210. doi:10.1007/s13205-018-1234-y.
  2. Jin, W., et al., Increased soluble heterologous expression of a rat brain 3-O-sulfotransferase 1 – A key enzyme for heparin biosynthesis. Protein Expression and Purification, 2018, Vol.151, P.23-29. doi: 10.1016/j.pep.2018.06.007
  3. Peng, Z., et al., High and efficient isomaltulose production using an engineered Yarrowia lipolytica strain. Bioresource Technology, June, 2018. doi: 10.1016/j.biortech.2018.06.081

引物合成

  1. Fang, B., et al., Luteolin inhibits angiogenesis of the M2‑like TAMs via the downregulation of hypoxia inducible factor‑1α and the STAT3 signalling pathway under hypoxia. Molecular Medicine Reports, July 3, 2018. doi: 10.3892/mmr.2018.9250.
  2. Chen, S., et al. Ubiquitin-conjugating enzyme UBE2O regulates cellular clock function by promoting the degradation of the transcription factor BMAL1. Journal of Biology Chemistry, 2018. doi: 10.1074/jbc.R A117.001432.
  3. Sun, Y., et al., Effect on the liver cancer cell invasion ability by studying the associations between autophagy and TRAP1 expression. Oncology Letters, 2018. doi: 10.3892/ol.2018.8774
  4. Wang, J., et al., Transcriptome profiling reveals PHLDA1 as a novel molecular marker for ischemic cardiomyopathy. Journal of Molecular Neuroscience, 2018. 65(1): p. 102-109. doi: 10.1007/s12031-018-1066-6.
  5. Zhang, H., et al., Genetic polymorphism in the RYR1 C6487T Is associated with severity of hypospadias in Chinese Han Children. BioMed Research International, 2018. Article ID 7397839. doi: 10.1155/2018/7397839.
  6. Wang, J., et al., Urban particulate matter triggers lung inflammation via the ROS-MAPK-NF-κB signaling pathway. Journal of Thoracic Disease, 2017. 9(11): p. 4398-4412. doi: 10.21037/jtd.2017.09.135.
  7. Yu, D., et al., Quercetin inhibits epithelial-mesenchymal transition, decreases invasiveness and metastasis, and reverses IL-6 induced epithelial-mesenchymal transition, expression of MMP by inhibiting STAT3 signaling in pancreatic cancer cells. OncoTargets and Therapy, 2017. 10: p. 4719-4729. doi: 10.2147/OTT.S136840.
  8. Zhang, Z., et al., Non-invasive detection of gastric cancer relevant D-amino acids with luminescent DNA/silver nanoclusters. Nanoscale, 2017. 9(48): p. 19367-19373. doi: 10.1039/c7nr07337b.
  9. Zhang, Y., et al., Genetic and immune features of resectable malignant brainstem gliomas. Oncotarget, 2017. 8(47): p. 82571-82582. doi: 10.18632/oncotarget.19653.
  10. Wang, Q., et al., Physcion 8-O-β-glucopyranoside induces apoptosis, suppresses invasion and inhibits epithelial to mesenchymal transition of hepatocellular carcinoma HepG2 cells. Biomedicine & Pharmacotherapy, 2016. 83: p. 372-380. doi:10.1016/j.biopha.2016.06.045.

探针合成

  1. Eastwood, M., et al., MicroRNA 200b is upregulated in the lungs of fetal rabbits with surgically induced diaphragmatic hernia. Prenatal Diagnosis, 2018. doi: 10.1002/pd.5318.
  2. Wu, Y., et al. A cyclic catalysis enhanced electrochemiluminescence aptasensor based 3D graphene/photocatalysts Cu2O-MWCNTs. Electochimica Acta, 2018. 282: p. 672-679. doi:10.1016/ j.electacta.2018.06.104.
  3. Zhang, Y., et al., Genetic and immune features of resectable malignant brainstem gliomas. Oncotarget, 2017. 8(47): p. 82571-82582. doi: 10. 18632/oncotarget.19653.

测序

  1. Chen, M., et al., Molecular cloning and characterization of C1 tetrahydrofolate (C1-THF) synthase in Bombyx mori, silkworm. Gene, 2018. 663: p. 25-33. doi:10.1016/j.gene.2018.04.037
  2. Yang, F., et al., Local polyploidy phenomenon in Escherichia coli and its significance in genetic engineering. BioRxiv, 2018. doi: 10.1101/335448
  3. Cheng, H., et al., The hyperthermophilic α-amylase from Thermococcus sp. HJ21 does not require exogenous calcium for thermostability because of high-binding affinity to calcium. Journal of Microbiology, 2017. 55(5): p. 379-387.doi: 10.1007/s12275-017-6416-5.

载体构建

  1. Chu J., et al., MiR-4319 Suppress the Malignancy of Triple-Negative Breast Cancer by Regulating Self-Renewal and Tumorigenesis of Stem Cells. Cell Physiol Biochem. 18th Jul 2018. 48(2): p.593-604. doi: 10.1159/000491888.
  2. Bellido, F., et al., Association between germline mutations in BRF1, a subunit of the RNA polymerase III transcription complex, and hereditary colorectal cancer. Gastroenterology, 2018. 154(1): p. 181-194. doi: 10.1053/j.gastro.2017.09.005.
  3. Xu, X., et al., Downregulation of HuR inhibits the progression of esophageal cancer through interleukin-18. Cancer Res Treat, 2018. 50(1): p.71-87. doi: 10.4143/crt.2017.013.

DNA存储

  1. Zhong, C., et al., A new digital information storing and reading system based on synthetic DNA. Science China. Life Sciences, 2018. 6: p. 1-3. doi: 10.1007/s11427-017-9131-7.

RNA干扰

  1. Ren, Y., et al., Role of c-Abl- GSK3β signaling in MPP+-induced autophagy-lysosomal dysfunction. Toxicological Sciences, 2018. kfy155. doi:10.1093/toxsci/kfy155.

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