客户科研成果列表


基因合成 | 基因克隆 | sgRNA文库 | 密码子优化| 引物合成| 探针合成| 测序| 载体构建| DNA存储| RNA干扰

基因合成

  1. Peng C., et al. Selective carboxylation of substituted phenols with engineered Escherichia coli whole-cells. Tetrahedron Letters, September, 2018. doi: 10.1016/j.tetlet.2018.09.019.
  2. Jian S., et al. Determining Allele-Specific Protein Expression (ASPE) Using a Novel QconCAT-Based Proteomics Method. Journal of Proteome Research, August 24th, 2018. doi: 10.1021/acs.jproteome.8b00620.
  3. Jinghua W., et al. CAR-T cells targeting CLL-1 as an approach to treat acute myeloid leukemia. Journal of Hematology & Oncology, 2018. 11:7. Doi: 10.1186/s13045-017-0553-5.
  4. Gong S., Wu C. Generation of Fabs-in-Tandem Immunoglobulin Molecules for Dual-Specific Targeting. Methods, Aug 3, 2018. pii: S1046-2023(18)30105-1. doi: 10.1016/j.ymeth.2018.07.014.
  5. Xiu, H., et al., microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147. Journal of Cancer. 2018; 9(15):2603-2611. doi:10.7150/jca.24918.
  6. Yang, X., et al., Expression of phosphotransacetylase in Rhodosporidium toruloides leading to improved cell growth and lipid production. Royal Society of Chemistry, 10th July 2018. 8:24673-24678. doi: 10.1039/C8RA03028F.
  7. Shi, J., et al., A novel QconCAT-based proteomics method for determining allele-specific protein expression (ASPE): a new approach to identify cis-acting genetic variants. BioRxiv, 2018. doi: 10.1101/264614.
  8. Su, J., et al., Galectin-10: a new structural type of prototype galectin dimer and effects on saccharide ligand binding. Glycobiology, 2018. 28(3): p. 159-168. doi:0.1093/glycob/cwx107
  9. Wang, X., et al., Visualizing soluble protein mutants by using monomeric red fluorescent protein as a reporter for directed evolution. Applied Biochemistry and Biotechnology, 2018. 185(1): p.81-90. doi: 10.1007/ s12010-017-2640-z.
  10. Lu, H., et al., Identification of a L-Lactate dehydrogenase with 3, 4-dihydroxyphenylpyruvic reduction activity for L-Danshensu production. Process Biochemistry, 2018. doi: 10.1016/j.procbio.2018.06.011
  11. Jin, W., et al., Increased soluble heterologous expression of a rat brain 3-O-sulfotransferase 1 – A key enzyme for heparin biosynthesis. Protein Expression and Purification, 2018, Vol.151, P.23-29. doi: 10.1016/j.pep.2018.06.007
  12. Li J, Zhang Y, Zhang Y, et al. GSNOR modulates hyperhomocysteinemia-induced T cell activation and atherosclerosis by switching Akt S-nitrosylation to phosphorylation[J]. Redox biology, 2018. doi.org/10.1016/j.redox.2018.04.021
  13. Cheng Y, Chen G, Wang L, et al. Triptolide-induced mitochondrial damage dysregulates fatty acid metabolism in mouse sertoli cell s[J]. Toxicology letters, 2018, 292: 136-150. doi: 10.1016/j.toxlet.2018.04.035.
  14. Pan Y, Sun H, Hu X, et al. The inhibitory role of miR 485 5p in colorectal cancer proliferation and invasion via targeting of CD147 [J]. Oncology reports, 2018, 39(5): 2201-2208. doi: 10.3892/or.2018.6309.
  15. Zhang W, He Z, Yi L, et al. A dual-functional microfluidic chip for on-line detection of interleukin-8 based on rolling circle amplification[J]. Biosensors and Bioelectronics, 2018, 102: 652-660. doi: 10.1016/j.bios.2017.12.017.
  16. Chang Z, Meng F, Zhang Z, et al. MicroRNA‐193b‐3p regulates matrix metalloproteinase 19 expression in interleukin‐1β‐induced human chondrocytes[J]. Journal of cellular biochemistry, 2018, 119(6): 4775-4782. doi: 10.1002/jcb.26669.
  17. He, P., et al., Development and application of a duplex real-time RT-PCR assay for simultaneous detection of coxsackievirus A6 and coxsackievirus A10. Annals of Clinical and Laboratory Science, 2017. 47(2): p. 178-183. PMID: 28442520
  18. Wang, Li., et al., Increased processivity, misincorporation, and nucleotide incorporation efficiency in Sulfolobus solfataricus Dpo4 thumb domain mutants. Applied and Environmental Microbiology, 2017. 83(18): p. e01013-17. doi: 10.1128/AEM.01013-17.
  19. Wang, X., et al., Engineering Escherichia coli nicotinic acid mononucleotide adenylyltransferase for fully active amidated NAD biosynthesis. Applied and Environmental Microbiology, 2017. 83(13): p. e00692-17. doi: 10.1128/AEM.00692-17.
  20. Xie, Z., et al., Design and chemical synthesis of eukaryotic chromosomes. Chemical Society Reviews, 2017. 46(23): p. 7191-7207. doi:10.1039/ c7cs00208ds
  21. Dai, S., et al., HNRNPA2B1 regulates the epithelial-mesenchymal transition in pancreatic cancer cells through the ERK/snail signalling pathway. Cancer Cell Int, 2017. 17: p. 12. doi: 10.1186/s12935-016-0368-4.
  22. Jin, L., et al., Icaritin induces mitochondrial apoptosis by up-regulating miR-124 in human oral squamous cell carcinoma cells. Biomed Pharmacother, 2017. 85: p. 287-295. doi: 10.1016/j.biopha.2016.11.023.
  23. Li, N., et al., A DNA-directed covalent conjugation fluorescence probe for in vitro detection of functional matrix metalloproteinases. Analyst, 2017. doi: 10.1039/C6AN02339H
  24. Liu J, Chen W, Zhang H, et al. miR 214 targets the PTEN mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer[J]. Oncology letters, 2017, 14(5): 5711-5718. doi: 10.3892/ol.2017.6953
  25. Duan L, Ding W, Liu X, et al. Biosynthesis and engineering of kaempferol in Saccharomyces cerevisiae[J]. Microbial cell factories, 2017, 16(1): 165. doi: 10.1186/s12934-017-0774-x
  26. Zhao Z, Xue J, Song Y, et al. Downregulated long non coding RNA TCONS_00068220 upregulates apoptosis in gastric cancer cells[J]. Oncology letters, 2017, 14(5): 6143-6150. doi: 10.3892/ol.2017.6977
  27. Si, X., et al., Upregulation of miR-99a is associated with poor prognosis of acute myeloid leukemia and promotes myeloid leukemia cell expansion. Oncotarget, 2016. 7(47): p. 78095-78109. doi: 10.18632/ oncotarget.12947.
  28. Na, R., et al., Indentification of vincamine indole alkaloids producing endophytic fungi isolated from Nerium indicum, Apocynaceae. Microbiol Res, 2016. 192: p. 114-21. doi: 10.1016/j.micres.2016.06.008.
  29. Pan, X., et al., Physcion induces apoptosis in hepatocellular carcinoma by modulating miR-370. Am J Cancer Res, 2016. 6(12): p. 2919-2931. PMCID: PMC5199765
  30. Li, Z., et al., NS5ATP6 modulates intracellular triglyceride content through FGF21 and independently of SIRT1 and SREBP1. Biochem Biophys Res Commun, 2016. 475(1): p. 133-9. doi: 10.1016/j.bbrc.2016.05.056.
  31. Yang, H., et al., Optimized expression of prolyl aminopeptidase in Pichia pastoris and its characteristics after glycosylation. World J Microbiol Biotechnol, 2016. 32(11): p. 176. doi: 10.1007/s11274-016-2135-z.
  32. Tang, Y., et al., Radiation-induced miR-208a increases the proliferation and radioresistance by targeting p21 in human lung cancer cells. J Exp Clin Cancer Res, 2016. 35: p. 7.doi: 10.1186/s13046-016-0285-3
  33. Zhi N, Wan Z, Liu X, et al. Codon optimization of human parvovirus B19 capsid genes greatly increases their expression in nonpermissive cells[J]. Journal of virology, 2010, 84(24): 13059-13062.doi: 10.1128/JVI.00912-10

基因克隆

  1. Hu, J., et al., Clone and functional analysis of Seryl-tRNA synthetase and Tyrosyl-tRNA synthetase from silkworm, Bombyx mori. Sci Rep, 2017. 7: p. 41563. doi: 10.1038/srep41563.
  2. Yan, H., et al., CRIF1 enhances p53 activity via the chromatin remodeler SNF5 in the HCT116 colon cancer cell lines. BBA – Gene Regulatory Mechanisms.doi: 10.1016/j.bbagrm

sgRNA文库

  1. Lu, Y., et al., Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System. Mol Plant, 2017.doi: 10.1016/j.molp.2017.06.007.

密码子优化

  1. Liu, B., et al., Codon optimization significantly enhanced the expression of human 37-kDa iLRP in Escherichia coli. Biotech, 2018. 8(4): p. 210. doi:10.1007/s13205-018-1234-y.
  2. Jin, W., et al., Increased soluble heterologous expression of a rat brain 3-O-sulfotransferase 1 – A key enzyme for heparin biosynthesis. Protein Expression and Purification, 2018, Vol.151, P.23-29. doi: 10.1016/j.pep.2018.06.007
  3. Peng, Z., et al., High and efficient isomaltulose production using an engineered Yarrowia lipolytica strain. Bioresource Technology, June, 2018. doi: 10.1016/j.biortech.2018.06.081

引物合成

  1. Haiying N., et al., Role of miR-214 in modulating proliferation and invasion of human colon cancer SW620 cells. Oncology Letters, September 28th, 2018. doi: 10.3892/ol.2018.9521.
  2. Jian W., et al., Amphiregulin potentiates airway inflammation and mucus hypersecretion induced by urban particulate matter via the EGFR-PI3Kα-AKT/ERK pathway. Cellular Signalling, January, 2019. doi: 10.1016/j.cellsig.2018.10.002.
  3. Fang, B., et al., Luteolin inhibits angiogenesis of the M2‑like TAMs via the downregulation of hypoxia inducible factor‑1α and the STAT3 signalling pathway under hypoxia. Molecular Medicine Reports, July 3, 2018. doi: 10.3892/mmr.2018.9250.
  4. Chen, S., et al. Ubiquitin-conjugating enzyme UBE2O regulates cellular clock function by promoting the degradation of the transcription factor BMAL1. Journal of Biology Chemistry, 2018. doi: 10.1074/jbc.R A117.001432.
  5. Sun, Y., et al., Effect on the liver cancer cell invasion ability by studying the associations between autophagy and TRAP1 expression. Oncology Letters, 2018. doi: 10.3892/ol.2018.8774
  6. Wang, J., et al., Transcriptome profiling reveals PHLDA1 as a novel molecular marker for ischemic cardiomyopathy. Journal of Molecular Neuroscience, 2018. 65(1): p. 102-109. doi: 10.1007/s12031-018-1066-6.
  7. Zhang, H., et al., Genetic polymorphism in the RYR1 C6487T Is associated with severity of hypospadias in Chinese Han Children. BioMed Research International, 2018. Article ID 7397839. doi: 10.1155/2018/7397839.
  8. Wang, J., et al., Urban particulate matter triggers lung inflammation via the ROS-MAPK-NF-κB signaling pathway. Journal of Thoracic Disease, 2017. 9(11): p. 4398-4412. doi: 10.21037/jtd.2017.09.135.
  9. Yu, D., et al., Quercetin inhibits epithelial-mesenchymal transition, decreases invasiveness and metastasis, and reverses IL-6 induced epithelial-mesenchymal transition, expression of MMP by inhibiting STAT3 signaling in pancreatic cancer cells. OncoTargets and Therapy, 2017. 10: p. 4719-4729. doi: 10.2147/OTT.S136840.
  10. Zhang, Z., et al., Non-invasive detection of gastric cancer relevant D-amino acids with luminescent DNA/silver nanoclusters. Nanoscale, 2017. 9(48): p. 19367-19373. doi: 10.1039/c7nr07337b.
  11. Zhang, Y., et al., Genetic and immune features of resectable malignant brainstem gliomas. Oncotarget, 2017. 8(47): p. 82571-82582. doi: 10.18632/oncotarget.19653.
  12. Wang, Q., et al., Physcion 8-O-β-glucopyranoside induces apoptosis, suppresses invasion and inhibits epithelial to mesenchymal transition of hepatocellular carcinoma HepG2 cells. Biomedicine & Pharmacotherapy, 2016. 83: p. 372-380. doi:10.1016/j.biopha.2016.06.045.

探针合成

  1. Eastwood, M., et al., MicroRNA 200b is upregulated in the lungs of fetal rabbits with surgically induced diaphragmatic hernia. Prenatal Diagnosis, 2018. doi: 10.1002/pd.5318.
  2. Wu, Y., et al. A cyclic catalysis enhanced electrochemiluminescence aptasensor based 3D graphene/photocatalysts Cu2O-MWCNTs. Electochimica Acta, 2018. 282: p. 672-679. doi:10.1016/ j.electacta.2018.06.104.
  3. Zhang, Y., et al., Genetic and immune features of resectable malignant brainstem gliomas. Oncotarget, 2017. 8(47): p. 82571-82582. doi: 10. 18632/oncotarget.19653.

测序

  1. Chen, M., et al., Molecular cloning and characterization of C1 tetrahydrofolate (C1-THF) synthase in Bombyx mori, silkworm. Gene, 2018. 663: p. 25-33. doi:10.1016/j.gene.2018.04.037
  2. Yang, F., et al., Local polyploidy phenomenon in Escherichia coli and its significance in genetic engineering. BioRxiv, 2018. doi: 10.1101/335448
  3. Cheng, H., et al., The hyperthermophilic α-amylase from Thermococcus sp. HJ21 does not require exogenous calcium for thermostability because of high-binding affinity to calcium. Journal of Microbiology, 2017. 55(5): p. 379-387.doi: 10.1007/s12275-017-6416-5.

载体构建

  1. Chu J., et al., MiR-4319 Suppress the Malignancy of Triple-Negative Breast Cancer by Regulating Self-Renewal and Tumorigenesis of Stem Cells. Cell Physiol Biochem. 18th Jul 2018. 48(2): p.593-604. doi: 10.1159/000491888.
  2. Bellido, F., et al., Association between germline mutations in BRF1, a subunit of the RNA polymerase III transcription complex, and hereditary colorectal cancer. Gastroenterology, 2018. 154(1): p. 181-194. doi: 10.1053/j.gastro.2017.09.005.
  3. Xu, X., et al., Downregulation of HuR inhibits the progression of esophageal cancer through interleukin-18. Cancer Res Treat, 2018. 50(1): p.71-87. doi: 10.4143/crt.2017.013.

DNA存储

  1. Zhong, C., et al., A new digital information storing and reading system based on synthetic DNA. Science China. Life Sciences, 2018. 6: p. 1-3. doi: 10.1007/s11427-017-9131-7.

RNA干扰

  1. Ren, Y., et al., Role of c-Abl- GSK3β signaling in MPP+-induced autophagy-lysosomal dysfunction. Toxicological Sciences, 2018. kfy155. doi:10.1093/toxsci/kfy155.

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