NGS捕获测序Blocking引物通常用于捕获测序中阻止捕获探针与文库adapter区的杂交,最小化捕获探针的脱靶效应,提高捕获测序的特异性。

Syno®Universal Blockers,是预先合成的Blocking引物,适用于使用Illumina®barcoded adapters构建的文库捕获,可以有效的提高用dual- or single-index adapters 构建的文库的捕获效率。

  1. 通过减少adapter与adapter的杂交提高捕获效率;
  2. 一对引物可以用于多种barcoded adapters文库;
  3. 预先合成引物减少等待时间和繁琐的操作。

target capture

Syno®Universal Blockers序列信息

TS-P5 Universal Blocker AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
TS-P7. 8nt Index 13 Blocker CAAGCAGAAGACGGCATACGAGATTGTTGACTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 14 Blocker CAAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 15 Blocker CAAGCAGAAGACGGCATACGAGATTCTGACATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 16 Blocker CAAGCAGAAGACGGCATACGAGATCGGGACGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 18 Blocker CAAGCAGAAGACGGCATACGAGATGTGCGGACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 19 Blocker CAAGCAGAAGACGGCATACGAGATCGTTTCACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 20 Blocker CAAGCAGAAGACGGCATACGAGATAAGGCCACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 21 Blocker CAAGCAGAAGACGGCATACGAGATTCCGAAACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 22 Blocker CAAGCAGAAGACGGCATACGAGATTACGTACGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 23 Blocker CAAGCAGAAGACGGCATACGAGATATCCACTCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 25 Blocker CAAGCAGAAGACGGCATACGAGATATATCAGTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
TS-P7. 8nt Index 27 Blocker CAAGCAGAAGACGGCATACGAGATAAAGGAATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT

 

TS-HT-i5-D501 Blocker AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCGGTGGTCGCCGTATCATT
TS-HT-i5-D502 Blocker AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTCGGTGGTCGCCGTATCATT
TS-HT-i5-D503 Blocker AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTCGGTGGTCGCCGTATCATT
TS-HT-i5-D504 Blocker AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTCGGTGGTCGCCGTATCATT
TS-HT-i5-D505 Blocker AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTCGGTGGTCGCCGTATCATT
TS-HT-i5-D506 Blocker AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTCGGTGGTCGCCGTATCATT
TS-HT-i5-D507 Blocker AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTCGGTGGTCGCCGTATCATT
TS-HT-i5-D508 Blocker AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTCGGTGGTCGCCGTATCATT
TS-HT-i7-D701 Blocker CAAGCAGAAGACGGCATACGAGATCGAGTAATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D702 Blocker CAAGCAGAAGACGGCATACGAGATTCTCCGGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D703 Blocker CAAGCAGAAGACGGCATACGAGATAATGAGCGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D704 Blocker CAAGCAGAAGACGGCATACGAGATGGAATCTCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D705 Blocker CAAGCAGAAGACGGCATACGAGATTTCTGAATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D706 Blocker CAAGCAGAAGACGGCATACGAGATACGAATTCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D707 Blocker CAAGCAGAAGACGGCATACGAGATAGCTTCAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D708 Blocker CAAGCAGAAGACGGCATACGAGATGCGCATTAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D709 Blocker CAAGCAGAAGACGGCATACGAGATCATAGCCGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D710 Blocker CAAGCAGAAGACGGCATACGAGATTTCGCGGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D711 Blocker CAAGCAGAAGACGGCATACGAGATGCGCGAGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC
TS-HT-i7-D712 Blocker CAAGCAGAAGACGGCATACGAGATCTATCGCTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC

服务价格与周期

规格 价格 周期
16 nmole Syno®Universal Blockers(HLPC), 16 rxn ¥3200/tube 次日发货
96 nmole Syno®Universal Blockers(HLPC), 96 rxn ¥19200/tube 次日发货

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